Construct: shRNA TRCN0000379154

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133981.1-661s21c1
DNA Barcode:
CGCCATGACGGGATGGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alg9 (102580)
Gene Description:
asparagine-linked glycosylation 9 (alpha 1,2 mannosyltransferase)
Transcript:
RefSeq NM_133981.1 (NON-CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379154)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCATGACGGGATGGTATAT
Hairpin Sequence:
5'-CCGG-CGCCATGACGGGATGGTATAT-CTCGAG-ATATACCATCCCGTCATGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCATGACGGGATGGTATATCTCGAGATATACCATCCCGTCATGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCATGACGGGATGGTATATCTCGAGATATACCATCCCGTCATGGCG-3'

Other clones with same target sequence:

(none)