Construct: shRNA TRCN0000379470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004408.2-1100s21c1
DNA Barcode:
TAGACTTTGAGAAGCGCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNM1 (1759)
Gene Description:
dynamin 1
Transcript:
RefSeq NM_004408.2 (NON-CURRENT)
Match location:
Position 1100 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGACTTTGAGAAGCGCATTG
Hairpin Sequence:
5'-CCGG-TAGACTTTGAGAAGCGCATTG-CTCGAG-CAATGCGCTTCTCAAAGTCTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGACTTTGAGAAGCGCATTGCTCGAGCAATGCGCTTCTCAAAGTCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGACTTTGAGAAGCGCATTGCTCGAGCAATGCGCTTCTCAAAGTCTA-3'

Other clones with same target sequence:

(none)