Construct: shRNA TRCN0000379521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080737.2-2854s21c1
DNA Barcode:
TCTTTCTCCTCTCCAATTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYTL4 (94121)
Gene Description:
synaptotagmin like 4
Transcript:
RefSeq NM_080737.2 (NON-CURRENT)
Match location:
Position 2854 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTTCTCCTCTCCAATTTAT
Hairpin Sequence:
5'-CCGG-TCTTTCTCCTCTCCAATTTAT-CTCGAG-ATAAATTGGAGAGGAGAAAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTTCTCCTCTCCAATTTATCTCGAGATAAATTGGAGAGGAGAAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTTCTCCTCTCCAATTTATCTCGAGATAAATTGGAGAGGAGAAAGA-3'

Other clones with same target sequence:

(none)