Construct: shRNA TRCN0000379525

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030906.2-1265s21c1
DNA Barcode:
AGCAGTCTTATTGATGATAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK33 (65975)
Gene Description:
serine/threonine kinase 33
Transcript:
RefSeq NM_030906.2 (NON-CURRENT)
Match location:
Position 1265 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379525)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGTCTTATTGATGATAAC
Hairpin Sequence:
5'-CCGG-AGCAGTCTTATTGATGATAAC-CTCGAG-GTTATCATCAATAAGACTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGTCTTATTGATGATAACCTCGAGGTTATCATCAATAAGACTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGTCTTATTGATGATAACCTCGAGGTTATCATCAATAAGACTGCT-3'

Other clones with same target sequence:

(none)