Construct: shRNA TRCN0000379538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018206.4-534s21c1
DNA Barcode:
GGGTCTGTTTCTTCGAAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS35 (55737)
Gene Description:
VPS35 retromer complex component
Transcript:
RefSeq NM_018206.4 (NON-CURRENT)
Match location:
Position 534 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTCTGTTTCTTCGAAATTA
Hairpin Sequence:
5'-CCGG-GGGTCTGTTTCTTCGAAATTA-CTCGAG-TAATTTCGAAGAAACAGACCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTCTGTTTCTTCGAAATTACTCGAGTAATTTCGAAGAAACAGACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTCTGTTTCTTCGAAATTACTCGAGTAATTTCGAAGAAACAGACCC-3'

Other clones with same target sequence:

(none)