Construct: shRNA TRCN0000379548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178815.3-579s21c1
DNA Barcode:
ATGTTGGCTCATGAGGATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARL5B (221079)
Gene Description:
ADP ribosylation factor like GTPase 5B
Transcript:
RefSeq NM_178815.3 (NON-CURRENT)
Match location:
Position 579 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTTGGCTCATGAGGATTTA
Hairpin Sequence:
5'-CCGG-ATGTTGGCTCATGAGGATTTA-CTCGAG-TAAATCCTCATGAGCCAACAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTTGGCTCATGAGGATTTACTCGAGTAAATCCTCATGAGCCAACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTTGGCTCATGAGGATTTACTCGAGTAAATCCTCATGAGCCAACAT-3'

Other clones with same target sequence:

(none)