Construct: shRNA TRCN0000379685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018208.2-1316s21c1
DNA Barcode:
ACGTGCAAGTCAACAAGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETNK2 (55224)
Gene Description:
ethanolamine kinase 2
Transcript:
RefSeq NM_018208.2 (NON-CURRENT)
Match location:
Position 1316 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGCAAGTCAACAAGTTTG
Hairpin Sequence:
5'-CCGG-ACGTGCAAGTCAACAAGTTTG-CTCGAG-CAAACTTGTTGACTTGCACGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGCAAGTCAACAAGTTTGCTCGAGCAAACTTGTTGACTTGCACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGCAAGTCAACAAGTTTGCTCGAGCAAACTTGTTGACTTGCACGT-3'

Other clones with same target sequence:

(none)