Construct: shRNA TRCN0000379691
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_028758.2-2160s21c1
- DNA Barcode:
- TCATCTCTGCTGCACTCATAC
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gga2 (74105)
- Gene Description:
- golgi associated, gamma adaptin ear containing, ARF binding protein 2
- Transcript:
- RefSeq NM_028758.2 (CURRENT)
- Match location:
- Position 2160 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000379691)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCATCTCTGCTGCACTCATAC
- Hairpin Sequence:
-
5'-CCGG-TCATCTCTGCTGCACTCATAC-CTCGAG-GTATGAGTGCAGCAGAGATGA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCATCTCTGCTGCACTCATACCTCGAGGTATGAGTGCAGCAGAGATGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCATCTCTGCTGCACTCATACCTCGAGGTATGAGTGCAGCAGAGATGA-3'
Other clones with same target sequence:
(none)
