Construct: shRNA TRCN0000379808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011181.3-314s21c1
DNA Barcode:
AGAGCTAAGTGAAGCTATGAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyth2 (19158)
Gene Description:
cytohesin 2
Transcript:
RefSeq NM_011181.3 (CURRENT)
Match location:
Position 314 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCTAAGTGAAGCTATGAG
Hairpin Sequence:
5'-CCGG-AGAGCTAAGTGAAGCTATGAG-CTCGAG-CTCATAGCTTCACTTAGCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCTAAGTGAAGCTATGAGCTCGAGCTCATAGCTTCACTTAGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCTAAGTGAAGCTATGAGCTCGAGCTCATAGCTTCACTTAGCTCT-3'

Other clones with same target sequence:

(none)