Construct: shRNA TRCN0000379816

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005177.3-3154s21c1
DNA Barcode:
GGGTCCAGGAAGATGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V0A1 (535)
Gene Description:
ATPase H+ transporting V0 subunit a1
Transcript:
RefSeq NM_005177.3 (NON-CURRENT)
Match location:
Position 3154 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379816)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTCCAGGAAGATGATATTT
Hairpin Sequence:
5'-CCGG-GGGTCCAGGAAGATGATATTT-CTCGAG-AAATATCATCTTCCTGGACCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTCCAGGAAGATGATATTTCTCGAGAAATATCATCTTCCTGGACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTCCAGGAAGATGATATTTCTCGAGAAATATCATCTTCCTGGACCC-3'

Other clones with same target sequence:

(none)