Construct: shRNA TRCN0000379866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004871.2-157s21c1
DNA Barcode:
ATGGAAGACGCGACAGGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOSR1 (9527)
Gene Description:
golgi SNAP receptor complex member 1
Transcript:
RefSeq NM_004871.2 (NON-CURRENT)
Match location:
Position 157 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGAAGACGCGACAGGTATA
Hairpin Sequence:
5'-CCGG-ATGGAAGACGCGACAGGTATA-CTCGAG-TATACCTGTCGCGTCTTCCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGAAGACGCGACAGGTATACTCGAGTATACCTGTCGCGTCTTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGAAGACGCGACAGGTATACTCGAGTATACCTGTCGCGTCTTCCAT-3'

Other clones with same target sequence:

(none)