Construct: shRNA TRCN0000379927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139061.4-2039s21c1
DNA Barcode:
ACACGAGGGCTGGTTCATAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps54 (245944)
Gene Description:
VPS54 GARP complex subunit
Transcript:
RefSeq NM_139061.4 (NON-CURRENT)
Match location:
Position 2039 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACGAGGGCTGGTTCATAAG
Hairpin Sequence:
5'-CCGG-ACACGAGGGCTGGTTCATAAG-CTCGAG-CTTATGAACCAGCCCTCGTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACGAGGGCTGGTTCATAAGCTCGAGCTTATGAACCAGCCCTCGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACGAGGGCTGGTTCATAAGCTCGAGCTTATGAACCAGCCCTCGTGT-3'

Other clones with same target sequence:

(none)