Construct: shRNA TRCN0000379933
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_017919.2-1476s21c1
- DNA Barcode:
- TTGTTATCAGATAGCGAAATC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STX17 (55014)
- Gene Description:
- syntaxin 17
- Transcript:
- RefSeq NM_017919.2 (NON-CURRENT)
- Match location:
- Position 1476 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000379933)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TTGTTATCAGATAGCGAAATC
- Hairpin Sequence:
-
5'-CCGG-TTGTTATCAGATAGCGAAATC-CTCGAG-GATTTCGCTATCTGATAACAA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTTGTTATCAGATAGCGAAATCCTCGAGGATTTCGCTATCTGATAACAATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATTGTTATCAGATAGCGAAATCCTCGAGGATTTCGCTATCTGATAACAA-3'
Other clones with same target sequence:
(none)
