Construct: shRNA TRCN0000379933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017919.2-1476s21c1
DNA Barcode:
TTGTTATCAGATAGCGAAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STX17 (55014)
Gene Description:
syntaxin 17
Transcript:
RefSeq NM_017919.2 (NON-CURRENT)
Match location:
Position 1476 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTTATCAGATAGCGAAATC
Hairpin Sequence:
5'-CCGG-TTGTTATCAGATAGCGAAATC-CTCGAG-GATTTCGCTATCTGATAACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTTATCAGATAGCGAAATCCTCGAGGATTTCGCTATCTGATAACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTTATCAGATAGCGAAATCCTCGAGGATTTCGCTATCTGATAACAA-3'

Other clones with same target sequence:

(none)