Construct: shRNA TRCN0000379934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146194.3-575s21c1
DNA Barcode:
ACTCATTACAACTCATCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Picalm (233489)
Gene Description:
phosphatidylinositol binding clathrin assembly protein
Transcript:
RefSeq NM_146194.3 (NON-CURRENT)
Match location:
Position 575 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCATTACAACTCATCATTT
Hairpin Sequence:
5'-CCGG-ACTCATTACAACTCATCATTT-CTCGAG-AAATGATGAGTTGTAATGAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCATTACAACTCATCATTTCTCGAGAAATGATGAGTTGTAATGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCATTACAACTCATCATTTCTCGAGAAATGATGAGTTGTAATGAGT-3'

Other clones with same target sequence:

(none)