Construct: shRNA TRCN0000379945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002446.3-581s21c1
DNA Barcode:
TGCAGCTAGAGGAGATCATCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K10 (4294)
Gene Description:
mitogen-activated protein kinase kinase kinase 10
Transcript:
RefSeq NM_002446.3 (NON-CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAGCTAGAGGAGATCATCG
Hairpin Sequence:
5'-CCGG-TGCAGCTAGAGGAGATCATCG-CTCGAG-CGATGATCTCCTCTAGCTGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAGCTAGAGGAGATCATCGCTCGAGCGATGATCTCCTCTAGCTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAGCTAGAGGAGATCATCGCTCGAGCGATGATCTCCTCTAGCTGCA-3'

Other clones with same target sequence:

(none)