Construct: shRNA TRCN0000379953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014203.2-263s21c1
DNA Barcode:
TCCGACATCCGGAACTGTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP2A1 (160)
Gene Description:
adaptor related protein complex 2 subunit alpha 1
Transcript:
RefSeq NM_014203.2 (NON-CURRENT)
Match location:
Position 263 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGACATCCGGAACTGTAAG
Hairpin Sequence:
5'-CCGG-TCCGACATCCGGAACTGTAAG-CTCGAG-CTTACAGTTCCGGATGTCGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGACATCCGGAACTGTAAGCTCGAGCTTACAGTTCCGGATGTCGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGACATCCGGAACTGTAAGCTCGAGCTTACAGTTCCGGATGTCGGA-3'

Other clones with same target sequence:

(none)