Construct: shRNA TRCN0000379954
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_002346410.1-493s21c1
- DNA Barcode:
- GCAAATCAGTCTTTCCGGAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROCK1P1 (727758)
- Gene Description:
- Rho associated coiled-coil containing protein kinase 1 pseudogene 1
- Transcript:
- RefSeq XM_002346410.1 (NON-CURRENT)
- Match location:
- Position 493 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000379954)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAAATCAGTCTTTCCGGAAA
- Hairpin Sequence:
-
5'-CCGG-GCAAATCAGTCTTTCCGGAAA-CTCGAG-TTTCCGGAAAGACTGATTTGC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAAATCAGTCTTTCCGGAAACTCGAGTTTCCGGAAAGACTGATTTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAAATCAGTCTTTCCGGAAACTCGAGTTTCCGGAAAGACTGATTTGC-3'
Other clones with same target sequence:
(none)
