Construct: shRNA TRCN0000380046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016128.3-2545s21c1
DNA Barcode:
GTCAGACAAAGTGCCGGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COPG1 (22820)
Gene Description:
COPI coat complex subunit gamma 1
Transcript:
RefSeq NM_016128.3 (NON-CURRENT)
Match location:
Position 2545 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGACAAAGTGCCGGATAA
Hairpin Sequence:
5'-CCGG-GTCAGACAAAGTGCCGGATAA-CTCGAG-TTATCCGGCACTTTGTCTGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGACAAAGTGCCGGATAACTCGAGTTATCCGGCACTTTGTCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGACAAAGTGCCGGATAACTCGAGTTATCCGGCACTTTGTCTGAC-3'

Other clones with same target sequence:

(none)