Construct: shRNA TRCN0000380052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004824.3-1555s21c1
DNA Barcode:
GCGATGTGGTTTGGGCTAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDYL (9425)
Gene Description:
chromodomain Y like
Transcript:
RefSeq NM_004824.3 (NON-CURRENT)
Match location:
Position 1555 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATGTGGTTTGGGCTAATG
Hairpin Sequence:
5'-CCGG-GCGATGTGGTTTGGGCTAATG-CTCGAG-CATTAGCCCAAACCACATCGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATGTGGTTTGGGCTAATGCTCGAGCATTAGCCCAAACCACATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATGTGGTTTGGGCTAATGCTCGAGCATTAGCCCAAACCACATCGC-3'

Other clones with same target sequence:

(none)