Construct: shRNA TRCN0000380090

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002736.2-472s21c1
DNA Barcode:
GGCGTTCAATGCTCCAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAR2B (5577)
Gene Description:
protein kinase cAMP-dependent type II regulatory subunit beta
Transcript:
RefSeq NM_002736.2 (NON-CURRENT)
Match location:
Position 472 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380090)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGTTCAATGCTCCAGTAAT
Hairpin Sequence:
5'-CCGG-GGCGTTCAATGCTCCAGTAAT-CTCGAG-ATTACTGGAGCATTGAACGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGTTCAATGCTCCAGTAATCTCGAGATTACTGGAGCATTGAACGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGTTCAATGCTCCAGTAATCTCGAGATTACTGGAGCATTGAACGCC-3'

Other clones with same target sequence:

(none)