Construct: shRNA TRCN0000380110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014178.6-1000s21c1
DNA Barcode:
GGATTCGGCAGAGTTTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STXBP6 (29091)
Gene Description:
syntaxin binding protein 6
Transcript:
RefSeq NM_014178.6 (NON-CURRENT)
Match location:
Position 1000 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATTCGGCAGAGTTTGATTT
Hairpin Sequence:
5'-CCGG-GGATTCGGCAGAGTTTGATTT-CTCGAG-AAATCAAACTCTGCCGAATCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATTCGGCAGAGTTTGATTTCTCGAGAAATCAAACTCTGCCGAATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATTCGGCAGAGTTTGATTTCTCGAGAAATCAAACTCTGCCGAATCC-3'

Other clones with same target sequence:

(none)