Construct: shRNA TRCN0000380148

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002344.5-1480s21c1
DNA Barcode:
CTCAACACTGAGCCTCCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LTK (4058)
Gene Description:
leukocyte receptor tyrosine kinase
Transcript:
RefSeq NM_002344.5 (NON-CURRENT)
Match location:
Position 1480 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380148)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAACACTGAGCCTCCTTAT
Hairpin Sequence:
5'-CCGG-CTCAACACTGAGCCTCCTTAT-CTCGAG-ATAAGGAGGCTCAGTGTTGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAACACTGAGCCTCCTTATCTCGAGATAAGGAGGCTCAGTGTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAACACTGAGCCTCCTTATCTCGAGATAAGGAGGCTCAGTGTTGAG-3'

Other clones with same target sequence:

(none)