Construct: shRNA TRCN0000380164

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173028.3-5442s21c1
DNA Barcode:
GAACTGGAAGTTCACTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps13a (271564)
Gene Description:
vacuolar protein sorting 13A
Transcript:
RefSeq NM_173028.3 (NON-CURRENT)
Match location:
Position 5442 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380164)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTGGAAGTTCACTATTAT
Hairpin Sequence:
5'-CCGG-GAACTGGAAGTTCACTATTAT-CTCGAG-ATAATAGTGAACTTCCAGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTGGAAGTTCACTATTATCTCGAGATAATAGTGAACTTCCAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTGGAAGTTCACTATTATCTCGAGATAATAGTGAACTTCCAGTTC-3'

Other clones with same target sequence:

(none)