Construct: shRNA TRCN0000380210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014496.3-2426s21c1
DNA Barcode:
ACCATTCACAGGTCCACAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA6 (27330)
Gene Description:
ribosomal protein S6 kinase A6
Transcript:
RefSeq NM_014496.3 (NON-CURRENT)
Match location:
Position 2426 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCATTCACAGGTCCACAATA
Hairpin Sequence:
5'-CCGG-ACCATTCACAGGTCCACAATA-CTCGAG-TATTGTGGACCTGTGAATGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCATTCACAGGTCCACAATACTCGAGTATTGTGGACCTGTGAATGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCATTCACAGGTCCACAATACTCGAGTATTGTGGACCTGTGAATGGT-3'

Other clones with same target sequence:

(none)