Construct: shRNA TRCN0000380213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005738.3-710s21c1
DNA Barcode:
AGGTGGTCAGGAGAAATTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARL4A (10124)
Gene Description:
ADP ribosylation factor like GTPase 4A
Transcript:
RefSeq NM_005738.3 (NON-CURRENT)
Match location:
Position 710 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTGGTCAGGAGAAATTAAG
Hairpin Sequence:
5'-CCGG-AGGTGGTCAGGAGAAATTAAG-CTCGAG-CTTAATTTCTCCTGACCACCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTGGTCAGGAGAAATTAAGCTCGAGCTTAATTTCTCCTGACCACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTGGTCAGGAGAAATTAAGCTCGAGCTTAATTTCTCCTGACCACCT-3'

Other clones with same target sequence:

(none)