Construct: shRNA TRCN0000380214

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014035.2-635s21c1
DNA Barcode:
GGAGTCCTCCATGGGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX24 (28966)
Gene Description:
sorting nexin 24
Transcript:
RefSeq NM_014035.2 (NON-CURRENT)
Match location:
Position 635 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380214)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGTCCTCCATGGGATATTT
Hairpin Sequence:
5'-CCGG-GGAGTCCTCCATGGGATATTT-CTCGAG-AAATATCCCATGGAGGACTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGTCCTCCATGGGATATTTCTCGAGAAATATCCCATGGAGGACTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGTCCTCCATGGGATATTTCTCGAGAAATATCCCATGGAGGACTCC-3'

Other clones with same target sequence:

(none)