Construct: shRNA TRCN0000380328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001660.3-1148s21c1
DNA Barcode:
ATAGCTCTTGTGCAACCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARF4 (378)
Gene Description:
ADP ribosylation factor 4
Transcript:
RefSeq NM_001660.3 (NON-CURRENT)
Match location:
Position 1148 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAGCTCTTGTGCAACCTTTA
Hairpin Sequence:
5'-CCGG-ATAGCTCTTGTGCAACCTTTA-CTCGAG-TAAAGGTTGCACAAGAGCTAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAGCTCTTGTGCAACCTTTACTCGAGTAAAGGTTGCACAAGAGCTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAGCTCTTGTGCAACCTTTACTCGAGTAAAGGTTGCACAAGAGCTAT-3'

Other clones with same target sequence:

(none)