Construct: shRNA TRCN0000380365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031394.2-123s21c1
DNA Barcode:
GGAGCAAGACGCCATCTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sytl2 (83671)
Gene Description:
synaptotagmin-like 2
Transcript:
RefSeq NM_031394.2 (NON-CURRENT)
Match location:
Position 123 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGCAAGACGCCATCTTAAA
Hairpin Sequence:
5'-CCGG-GGAGCAAGACGCCATCTTAAA-CTCGAG-TTTAAGATGGCGTCTTGCTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGCAAGACGCCATCTTAAACTCGAGTTTAAGATGGCGTCTTGCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGCAAGACGCCATCTTAAACTCGAGTTTAAGATGGCGTCTTGCTCC-3'

Other clones with same target sequence:

(none)