Construct: shRNA TRCN0000380392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028238.7-345s21c1
DNA Barcode:
TGGAAACATGACAAGAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab38 (72433)
Gene Description:
RAB38, member RAS oncogene family
Transcript:
RefSeq NM_028238.7 (CURRENT)
Match location:
Position 345 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAAACATGACAAGAGTTTA
Hairpin Sequence:
5'-CCGG-TGGAAACATGACAAGAGTTTA-CTCGAG-TAAACTCTTGTCATGTTTCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAAACATGACAAGAGTTTACTCGAGTAAACTCTTGTCATGTTTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAAACATGACAAGAGTTTACTCGAGTAAACTCTTGTCATGTTTCCA-3'

Other clones with same target sequence:

(none)