Construct: shRNA TRCN0000380436

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011515.4-467s21c1
DNA Barcode:
GCACAACTGAAGCATCACTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vamp7 (20955)
Gene Description:
vesicle-associated membrane protein 7
Transcript:
RefSeq NM_011515.4 (NON-CURRENT)
Match location:
Position 467 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380436)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAACTGAAGCATCACTCT
Hairpin Sequence:
5'-CCGG-GCACAACTGAAGCATCACTCT-CTCGAG-AGAGTGATGCTTCAGTTGTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAACTGAAGCATCACTCTCTCGAGAGAGTGATGCTTCAGTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAACTGAAGCATCACTCTCTCGAGAGAGTGATGCTTCAGTTGTGC-3'

Other clones with same target sequence:

(none)