Construct: shRNA TRCN0000380440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002717.2-885s21c1
DNA Barcode:
GCAGATGATTTGCGGATTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R2A (5520)
Gene Description:
protein phosphatase 2 regulatory subunit Balpha
Transcript:
RefSeq NM_002717.2 (NON-CURRENT)
Match location:
Position 885 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGATTTGCGGATTAAT
Hairpin Sequence:
5'-CCGG-GCAGATGATTTGCGGATTAAT-CTCGAG-ATTAATCCGCAAATCATCTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGATTTGCGGATTAATCTCGAGATTAATCCGCAAATCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGATTTGCGGATTAATCTCGAGATTAATCCGCAAATCATCTGC-3'

Other clones with same target sequence:

(none)