Construct: shRNA TRCN0000380543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005997.1-670s21c1
DNA Barcode:
GCCCATAATCACCTATCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS72 (6944)
Gene Description:
vacuolar protein sorting 72 homolog
Transcript:
RefSeq NM_005997.1 (NON-CURRENT)
Match location:
Position 670 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATAATCACCTATCATTC
Hairpin Sequence:
5'-CCGG-GCCCATAATCACCTATCATTC-CTCGAG-GAATGATAGGTGATTATGGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATAATCACCTATCATTCCTCGAGGAATGATAGGTGATTATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATAATCACCTATCATTCCTCGAGGAATGATAGGTGATTATGGGC-3'

Other clones with same target sequence:

(none)