Construct: shRNA TRCN0000380562

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016281.3-890s21c1
DNA Barcode:
TGGACTAGCCTACCTACATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAOK3 (51347)
Gene Description:
TAO kinase 3
Transcript:
RefSeq NM_016281.3 (NON-CURRENT)
Match location:
Position 890 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380562)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGACTAGCCTACCTACATTC
Hairpin Sequence:
5'-CCGG-TGGACTAGCCTACCTACATTC-CTCGAG-GAATGTAGGTAGGCTAGTCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGACTAGCCTACCTACATTCCTCGAGGAATGTAGGTAGGCTAGTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGACTAGCCTACCTACATTCCTCGAGGAATGTAGGTAGGCTAGTCCA-3'

Other clones with same target sequence:

(none)