Construct: shRNA TRCN0000380701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172646.2-1432s21c1
DNA Barcode:
GGCGTTTGAGGCAATAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnm3 (103967)
Gene Description:
dynamin 3
Transcript:
RefSeq NM_172646.2 (CURRENT)
Match location:
Position 1432 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGTTTGAGGCAATAGTTAA
Hairpin Sequence:
5'-CCGG-GGCGTTTGAGGCAATAGTTAA-CTCGAG-TTAACTATTGCCTCAAACGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGTTTGAGGCAATAGTTAACTCGAGTTAACTATTGCCTCAAACGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGTTTGAGGCAATAGTTAACTCGAGTTAACTATTGCCTCAAACGCC-3'

Other clones with same target sequence:

(none)