Construct: shRNA TRCN0000380721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003826.2-582s21c1
DNA Barcode:
GAGCGAGCTGGAAAGCTTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAPG (8774)
Gene Description:
NSF attachment protein gamma
Transcript:
RefSeq NM_003826.2 (NON-CURRENT)
Match location:
Position 582 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380721)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGAGCTGGAAAGCTTATA
Hairpin Sequence:
5'-CCGG-GAGCGAGCTGGAAAGCTTATA-CTCGAG-TATAAGCTTTCCAGCTCGCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGAGCTGGAAAGCTTATACTCGAGTATAAGCTTTCCAGCTCGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGAGCTGGAAAGCTTATACTCGAGTATAAGCTTTCCAGCTCGCTC-3'

Other clones with same target sequence:

(none)