Construct: shRNA TRCN0000380752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017817.1-1028s21c1
DNA Barcode:
GCAGAATGTTGGAGTGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB20 (55647)
Gene Description:
RAB20, member RAS oncogene family
Transcript:
RefSeq NM_017817.1 (NON-CURRENT)
Match location:
Position 1028 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAATGTTGGAGTGGAAAT
Hairpin Sequence:
5'-CCGG-GCAGAATGTTGGAGTGGAAAT-CTCGAG-ATTTCCACTCCAACATTCTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAATGTTGGAGTGGAAATCTCGAGATTTCCACTCCAACATTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAATGTTGGAGTGGAAATCTCGAGATTTCCACTCCAACATTCTGC-3'

Other clones with same target sequence:

(none)