Construct: shRNA TRCN0000380812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001635.2-240s21c1
DNA Barcode:
GCGAGAACTCCGAGGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMPH (273)
Gene Description:
amphiphysin
Transcript:
RefSeq NM_001635.2 (NON-CURRENT)
Match location:
Position 240 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGAACTCCGAGGATATTT
Hairpin Sequence:
5'-CCGG-GCGAGAACTCCGAGGATATTT-CTCGAG-AAATATCCTCGGAGTTCTCGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGAACTCCGAGGATATTTCTCGAGAAATATCCTCGGAGTTCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGAACTCCGAGGATATTTCTCGAGAAATATCCTCGGAGTTCTCGC-3'

Other clones with same target sequence:

(none)