Construct: shRNA TRCN0000380829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003931.2-1521s21c1
DNA Barcode:
TAATGGCCCAGCCTCTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WASF1 (8936)
Gene Description:
WASP family member 1
Transcript:
RefSeq NM_003931.2 (NON-CURRENT)
Match location:
Position 1521 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATGGCCCAGCCTCTCATTT
Hairpin Sequence:
5'-CCGG-TAATGGCCCAGCCTCTCATTT-CTCGAG-AAATGAGAGGCTGGGCCATTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATGGCCCAGCCTCTCATTTCTCGAGAAATGAGAGGCTGGGCCATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATGGCCCAGCCTCTCATTTCTCGAGAAATGAGAGGCTGGGCCATTA-3'

Other clones with same target sequence:

(none)