Construct: shRNA TRCN0000380880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004945.2-1533s21c1
DNA Barcode:
GAGCGAATCGTCACCACTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNM2 (1785)
Gene Description:
dynamin 2
Transcript:
RefSeq NM_004945.2 (NON-CURRENT)
Match location:
Position 1533 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGAATCGTCACCACTTAC
Hairpin Sequence:
5'-CCGG-GAGCGAATCGTCACCACTTAC-CTCGAG-GTAAGTGGTGACGATTCGCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGAATCGTCACCACTTACCTCGAGGTAAGTGGTGACGATTCGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGAATCGTCACCACTTACCTCGAGGTAAGTGGTGACGATTCGCTC-3'

Other clones with same target sequence:

(none)