Construct: shRNA TRCN0000380911

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006178.2-1938s21c1
DNA Barcode:
GTCCCTATTGGCCCTCGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NSF (4905)
Gene Description:
N-ethylmaleimide sensitive factor, vesicle fusing ATPase
Transcript:
RefSeq NM_006178.2 (NON-CURRENT)
Match location:
Position 1938 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380911)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCCTATTGGCCCTCGATTT
Hairpin Sequence:
5'-CCGG-GTCCCTATTGGCCCTCGATTT-CTCGAG-AAATCGAGGGCCAATAGGGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCCTATTGGCCCTCGATTTCTCGAGAAATCGAGGGCCAATAGGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCCTATTGGCCCTCGATTTCTCGAGAAATCGAGGGCCAATAGGGAC-3'

Other clones with same target sequence:

(none)