Construct: shRNA TRCN0000380931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019806.5-219s21c1
DNA Barcode:
GGCAACCCAACAGACCGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vapb (56491)
Gene Description:
vesicle-associated membrane protein, associated protein B and C
Transcript:
RefSeq NM_019806.5 (CURRENT)
Match location:
Position 219 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAACCCAACAGACCGAAAT
Hairpin Sequence:
5'-CCGG-GGCAACCCAACAGACCGAAAT-CTCGAG-ATTTCGGTCTGTTGGGTTGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAACCCAACAGACCGAAATCTCGAGATTTCGGTCTGTTGGGTTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAACCCAACAGACCGAAATCTCGAGATTTCGGTCTGTTGGGTTGCC-3'

Other clones with same target sequence:

(none)