Construct: shRNA TRCN0000380976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006990.2-1694s21c1
DNA Barcode:
AGGACGACTGGTCCGATTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WASF2 (10163)
Gene Description:
WASP family member 2
Transcript:
RefSeq NM_006990.2 (NON-CURRENT)
Match location:
Position 1694 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACGACTGGTCCGATTAAC
Hairpin Sequence:
5'-CCGG-AGGACGACTGGTCCGATTAAC-CTCGAG-GTTAATCGGACCAGTCGTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACGACTGGTCCGATTAACCTCGAGGTTAATCGGACCAGTCGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACGACTGGTCCGATTAACCTCGAGGTTAATCGGACCAGTCGTCCT-3'

Other clones with same target sequence:

(none)