Construct: shRNA TRCN0000380982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005188.2-2301s21c1
DNA Barcode:
TTGCGACCAGCAGATTGATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBL (867)
Gene Description:
Cbl proto-oncogene
Transcript:
RefSeq NM_005188.2 (NON-CURRENT)
Match location:
Position 2301 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCGACCAGCAGATTGATAG
Hairpin Sequence:
5'-CCGG-TTGCGACCAGCAGATTGATAG-CTCGAG-CTATCAATCTGCTGGTCGCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCGACCAGCAGATTGATAGCTCGAGCTATCAATCTGCTGGTCGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCGACCAGCAGATTGATAGCTCGAGCTATCAATCTGCTGGTCGCAA-3'

Other clones with same target sequence:

(none)