Construct: shRNA TRCN0000381039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_197996.2-1281s21c1
DNA Barcode:
GCCTTGGCCAGTGGTCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tspan15 (70423)
Gene Description:
tetraspanin 15
Transcript:
RefSeq NM_197996.2 (CURRENT)
Match location:
Position 1281 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGGCCAGTGGTCATTAT
Hairpin Sequence:
5'-CCGG-GCCTTGGCCAGTGGTCATTAT-CTCGAG-ATAATGACCACTGGCCAAGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGGCCAGTGGTCATTATCTCGAGATAATGACCACTGGCCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGGCCAGTGGTCATTATCTCGAGATAATGACCACTGGCCAAGGC-3'

Other clones with same target sequence:

(none)