Construct: shRNA TRCN0000381059

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016154.3-597s21c1
DNA Barcode:
CTCAAGTGTGCCCGCACTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB4B (53916)
Gene Description:
RAB4B, member RAS oncogene family
Transcript:
RefSeq NM_016154.3 (NON-CURRENT)
Match location:
Position 597 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381059)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAAGTGTGCCCGCACTATC
Hairpin Sequence:
5'-CCGG-CTCAAGTGTGCCCGCACTATC-CTCGAG-GATAGTGCGGGCACACTTGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAAGTGTGCCCGCACTATCCTCGAGGATAGTGCGGGCACACTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAAGTGTGCCCGCACTATCCTCGAGGATAGTGCGGGCACACTTGAG-3'

Other clones with same target sequence:

(none)