Construct: shRNA TRCN0000381076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004305.2-477s21c1
DNA Barcode:
TGAGCAGTGCGTCCAGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BIN1 (274)
Gene Description:
bridging integrator 1
Transcript:
RefSeq NM_004305.2 (NON-CURRENT)
Match location:
Position 477 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGCAGTGCGTCCAGAATTT
Hairpin Sequence:
5'-CCGG-TGAGCAGTGCGTCCAGAATTT-CTCGAG-AAATTCTGGACGCACTGCTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGCAGTGCGTCCAGAATTTCTCGAGAAATTCTGGACGCACTGCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGCAGTGCGTCCAGAATTTCTCGAGAAATTCTGGACGCACTGCTCA-3'

Other clones with same target sequence:

(none)