Construct: shRNA TRCN0000381208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011180.3-1669s21c1
DNA Barcode:
TGCTCCGAGAATGCCTGTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyth1 (19157)
Gene Description:
cytohesin 1
Transcript:
RefSeq NM_011180.3 (CURRENT)
Match location:
Position 1669 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTCCGAGAATGCCTGTAAG
Hairpin Sequence:
5'-CCGG-TGCTCCGAGAATGCCTGTAAG-CTCGAG-CTTACAGGCATTCTCGGAGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTCCGAGAATGCCTGTAAGCTCGAGCTTACAGGCATTCTCGGAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTCCGAGAATGCCTGTAAGCTCGAGCTTACAGGCATTCTCGGAGCA-3'

Other clones with same target sequence:

(none)