Construct: shRNA TRCN0000381249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004287.3-285s21c1
DNA Barcode:
ACCAGTTAAAGTATGATGTCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOSR2 (9570)
Gene Description:
golgi SNAP receptor complex member 2
Transcript:
RefSeq NM_004287.3 (NON-CURRENT)
Match location:
Position 285 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAGTTAAAGTATGATGTCC
Hairpin Sequence:
5'-CCGG-ACCAGTTAAAGTATGATGTCC-CTCGAG-GGACATCATACTTTAACTGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAGTTAAAGTATGATGTCCCTCGAGGGACATCATACTTTAACTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAGTTAAAGTATGATGTCCCTCGAGGGACATCATACTTTAACTGGT-3'

Other clones with same target sequence:

(none)