Construct: shRNA TRCN0000381307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016801.3-393s21c1
DNA Barcode:
GGAGGTCATGTCCGAGTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stx1a (20907)
Gene Description:
syntaxin 1A (brain)
Transcript:
RefSeq NM_016801.3 (NON-CURRENT)
Match location:
Position 393 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGGTCATGTCCGAGTACAA
Hairpin Sequence:
5'-CCGG-GGAGGTCATGTCCGAGTACAA-CTCGAG-TTGTACTCGGACATGACCTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGGTCATGTCCGAGTACAACTCGAGTTGTACTCGGACATGACCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGGTCATGTCCGAGTACAACTCGAGTTGTACTCGGACATGACCTCC-3'

Other clones with same target sequence:

(none)