Construct: shRNA TRCN0000381431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019535.2-1005s21c1
DNA Barcode:
GCCGAGCTCTGTATGACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3gl2 (20404)
Gene Description:
SH3-domain GRB2-like 2
Transcript:
RefSeq NM_019535.2 (CURRENT)
Match location:
Position 1005 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAGCTCTGTATGACTTTG
Hairpin Sequence:
5'-CCGG-GCCGAGCTCTGTATGACTTTG-CTCGAG-CAAAGTCATACAGAGCTCGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAGCTCTGTATGACTTTGCTCGAGCAAAGTCATACAGAGCTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAGCTCTGTATGACTTTGCTCGAGCAAAGTCATACAGAGCTCGGC-3'

Other clones with same target sequence:

(none)